| Immunostimulatory effects of BCG-CWS on the proliferation and viability of mouse spleen cells |
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Che-Wook Lee, Eun-Ju Ko, Hong-Gu Joo |
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Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University |
| 마우스 비장세포의 증식과 생존율에 대한 BCG-CWS의 면역자극 효과 |
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이제욱, 고은주, 주홍구 |
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제주대학교 수의과대학 수의약리학실험실 |
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| Abstract |
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Mycobacterial cell-wall skeleton (CWS) is an immunoactive and biodegradable particulate adjuvant and has been tried to use for immunotherapy. The CWS of Mycobacterium bovis bacillus Calmette-Guerin (BCG-CWS) was studied as an universal vaccine vehicle for antigen conjugation, to develop potentially effective and safe vaccine. Although a variety of biological activities of BCG-CWS have been studied, the effects of BCG-CWS on spleen cells are not fully elucidated. Using MTT assay and trypan blue exclusion test, we found that BCG-CWS significantly enhanced the viability and proliferation of cells. Multiple clusters, indicating proliferation, were observed in BCG-CWS-treated spleen cells and surface marker staining assay revealed that BCG-CWS promoted the proliferation of $CD19^+$ B lymphocyte rather than $CD4^+$ or $CD8^+$ T lymphocyte. In addition, BCG-CWS up-regulated the expression of anti-apoptotic molecules such as bcl-2, bcl-xL. BCG-CWS increased the surface expression of CD25 and CD69 as well as IL-2 production of spleen cells, suggesting increased activation. Furthermore, BCG-CWS enhanced the antigen-specific cell proliferation and interferon-gamma production of spleen cells. Taken together, these results demonstrate the immunostimulatory effects of BCG-CWS on spleen cells via multiple mechanisms, providing valuable information to broaden the use of BCG-CWS in clinical and research settings. |
| Key Words:
adjuvant, BCG-CWS, spleen cell, vaccine |
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