| The expression of Foxp3 protein by retroviral vector-mediated gene transfer of Foxp3 in C57BL/6 mice |
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Insun Hwang1, Danbee Ha1, So Jin Bing1, Kyong-Leek Jeon1, Ginnae Ahn2, Dae Seung Kim1, Jinhee Cho1, Jaehak Lim1, Sin-Hyeog Im3, Kyu-Kye Hwang1, Youngheun Jee1 |
1College of Veterinary Medicine and Applied Radiological Institute, Jeju National University
2Department of Marine Life Sciences, Jeju National University
3School of Life Sciences and Immune Synapse Research Center, Gwangju Institute of Science and Technology (GIST) |
| C57BL/6 마우스에서 Retroviral 벡터를 이용한 Foxp3 유전자의 도입에 의한 Foxp3 단백의 발현 양상 |
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황인선1, 하단비1, 빙소진1, 전경익1, 안긴내2, 김대승1, 조진희1, 임재학1, 임신혁3, 황규계1, 지영흔1 |
1제주대학교 수의과대학 수의학과
2제주대학교 해양과학대학 해양생명과학과
3광주과학기술원 생명과학부 면역조절연구실 |
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| Abstract |
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The maintenance of peripheral immune tolerance and prevention of chronic inflammation and autoimmune disease require $CD4^{+}CD25^{+}$ T cells (regulatory T cells). The transcription factor Foxp3 is essential for the development of functional, regulatory T cells, which plays a prominent role in self-tolerance. Retroviral vectors can confer high level of gene transfer and transgene expression in a variety of cell types. Here we observed that following retroviral vector-mediated gene transfer of Foxp3, transductional Foxp3 expression was increased in the liver, lung, brain, heart, muscle, spinal cord, kidney and spleen. One day after vector administration, high levels of transgene and gene expression were observed in liver and lung. At 2 days after injection, transductional Foxp3 expression level was increased in brain, heart, muscle and spinal cord, but kidney and spleen exhibited a consistent low level. This finding was inconsistent with the increase in both $CD4^{+}CD25^{+}$ T cell and $CD4^{+}Foxp3^{+}$ T cell frequencies observed in peripheral immune cells by fluorescence-activated cell-sorting (FACS) analysis. Retroviral vector-mediated gene transfer of Foxp3 did not lead to increased numbers of $CD4^{+}CD25^{+}$ T cell and $CD4^{+}Foxp3^{+}$ T cell. These results demonstrate the level and duration of transductional Foxp3 gene expression in various tissues. A better understanding of Foxp3 regulation can be useful in dissecting the cause of regulatory T cells dysfunction in several autoimmune diseases and raise the possibility of enhancing suppressive functions of regulatory T cells for therapeutic purposes. |
| Key Words:
Foxp3, regulatory T cells, retroviral vector, gene transfer |
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