An immunohistochemical study on the initial development of 155 kd neurofascin in rat sciatic nerve

Won, Hee-Young; Cho, Ik-Hyun; Lee, Jong-Hwan; Nahm, Sang-Soep; Choe, Nong-Hoon; Park, Jae-Hyung; Chang, Byung-Joon

doi:2012.52.3.199

Korean J Vet Res > Volume 52(3); 2012 > Article

Original Article

Korean Journal of Veterinary Research 2012;52(3):199-203.
DOI: https://doi.org/10.14405/kjvr.2012.52.3.199 Published online September 30, 2012.

An immunohistochemical study on the initial development of 155 kd neurofascin in rat sciatic nerve

Hee-Young Won¹, Ik-Hyun Cho², Jong-Hwan Lee¹, Sang-Soep Nahm¹, Nong-Hoon Choe¹, Jae-Hyung Park³, Byung-Joon Chang¹

¹College of Veterinary Medicine and Veterinary Research Institute, Konkuk University
²School of Medicine, College of Oriental Medicine, Kyunghee University
³Daewon International Middle School

랫드 궁둥신경에서 155 kd Neurofascin의 발현개시에 대한 면역세포화학적연구

원희영¹, 조익현², 이종환¹, 남상섭¹, 최농훈¹, 박재형³, 장병준¹

¹건국대학교 수의과대학 및 수의과학연구소
²경희대학교 한의과대학
³대원국제중학교

Abstract

Neurofascin, one of the members of L1CAM, has been known to have some important roles during the development of nerve fibers. In order to investigate the role of neurofascin associated with the development of nerve fibers in the rat sciatic nerve, the initial development of NF155 in the paranode was studied with immuno-fluorescence and immuno-electron microscopy. The result of the present study showed NF155 was not detected in the fetal sciatic nerve and began to reveal at the postnatal day 0 (P0) and dramatically increased by time lapse until postnatal day 7 (P7). NF155 was prominently localized in the axolemma of paranode and not detected in the central region of node of Ranvier. According to the present study, NF155 is likely to have some relationships with the formation of paranode and myelin sheath.

Key Words: immunocytochemistry, NF155, paranode, sciatic nerve