Korean J Vet Res > Volume 53(1); 2013 > Article
Korean Journal of Veterinary Research 2013;53(1):11-17.
DOI: https://doi.org/10.14405/kjvr.2013.53.1.011    Published online March 31, 2013.
Sequential hepatic ultrastructural changes and apoptosis in rabbits experimentally infected with Korean strain of rabbit hemorrhagic disease virus (RHDVa)
Jung-Won Park1, Ji-Eun Chun1, Eun-Jung Bak2, Han Kim1, Myeong-Heon Lee1, Eui-Kyung Hwang3, Jae-Hoon Kim4, Chung-Bok Lee5, Gye-Hyeong Woo6
1Animal, Plant and Fisheries Quarantine and Inspection Agency
2Oral Cancer Research Institute, College of Dentistry, Yonsei University
3Department of Animal Science, College of Life Science and Natural Resources, Sangji University
4College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University
5College of Veterinary Medicine, Konkuk University
6Department of Clinical Laboratory Science, Semyung University
국내 분리 토끼출혈병 바이러스(RHDVa)를 감염시킨 토끼 간장에서의 경시적인 초미세구조 변화와 apoptosis
박중원1, 전지은1, 박은정2, 김한1, 이명헌1, 황의경3, 김재훈4, 이중복5, 우계형6
2연세대학교 치과대학
3상지대학교 동물자원학과
4제주대학교 수의과대학
5건국대학교 수의과대학
6세명대학교 임상병리학과
In this study, to understand the pathogenesis of new rabbit hemorrhagic disease virus (RHDVa) serotype, we carried out to administrate RHDVa to rabbits, and to examine sequential electron microscopic changes and relationship between pathogenesis and apoptosis. TUNEL-positive cells began to be observed from 24 hours after inoculation (HAI) and the number of positive cells was slightly increased with the course of time. Whereas marked increase of positive cells was seen in the liver from the rabbits died acutely. Typical viral particles with cup-like projections and a diameter of 30~40 nm were detected in homogenized liver samples and tissues at 36 and 48, and 48 HAI, respectively. Ultrastructurally, glycogen deposition was observed from the first stage of hepatocellular degeneration by RHDVa infection and then, swelling and disruption of cristae of mitochondria by viral particles, swelling of smooth endoplasmic reticulum, vacuoles and vesicles were detected. Condensation, margination and fragmentation of chromatin were observed in degenerative hepatocytes at 36 and 48 HAI, indicating apoptotic bodies. These data offer that hepatocytic apoptosis by RHDV infection could be closely related with mitochondrial impairment in the hepatocytes.
Key Words: apoptosis, mitochondrial impairment, new RHDVa serotype, rabbit hemorrhagic disease, ultrastructure

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