| Application of SYBR Green real-time PCR assay for the specific detection of Salmonella spp. |
|
Seung Won Shin1, Seung Bin Cha1, Won-Jung Lee1, Min-Kyoung Shin1, Myunghwan Jung1, Anna Yoo1, Byeng Yeal Jung2, Han Sang Yoo1 |
1Department of Infectious Diseases, College of Veterinary Medicine and Brain Korea 21 Program for Veterinary Science, Seoul National University
2Bacterial Disease Division, Department of Animal and Plant Health Research, Animal, Plant and Fisheries Quarantine and Inspection Agency |
| Salmonella spp. 특이적인 검출을 위한 SYBR Green real-time PCR 기법 적용 |
|
신승원1, 차승빈1, 이원정1, 신민경1, 정명환1, 유안나1, 정병열2, 유한상1 |
1서울대학교 수의과대학 및 BK21 수의과학인력양성 사업단
2농림수산검역검사본부 동식물위생연구부 세균질병과 |
|
|
| Abstract |
|
The aim of this study was to applicate and evaluate a SYBR Green real-time PCR for the specific detection of Salmonella spp. Specificity of the PCR method was confirmed with 48 Salmonella spp. and 5 non-Salmonella strains using invA gene primer. The average threshold cycle ($C_T$) of Salmonella spp. was $11.83{pm}0.78$ while non-Salmonella spp. was $30.86{pm}1.19$. Correlation coefficients of standard curves constructed using $C_T$ versus copy number of Salmonella Enteritidis ATCC 13076 showed good linearity ($R^2=0.993$; slope = 3.563). Minimum level of detection with the method was > $10^2$ colony forming units (CFU)/mL. These results suggested that the SYBR Green real-time PCR might be applicable for the specific detection of Salmonella spp. isolates. |
| Key Words:
invA, Salmonella spp, specific detection, SYBR Green real-time PCR |
|
PDF Links
Full text via DOI
Download Citation
