Characterization of multipotent mesenchymal stem cells isolated from adipose tissue and bone marrow in pigs

Lee, Ah-Young; Choe, Gyeong-Im; Nah, Jin-Ju; So, ByungJae; Lee, Kyung-Woo; Chang, Ki-Yoon; Song, Jae-Young; Cha, Sang-Ho

doi:2013.53.1.37

Korean J Vet Res > Volume 53(1); 2013 > Article

Original Article

Korean Journal of Veterinary Research 2013;53(1):37-42.
DOI: https://doi.org/10.14405/kjvr.2013.53.1.037 Published online March 31, 2013.

Characterization of multipotent mesenchymal stem cells isolated from adipose tissue and bone marrow in pigs

Ah-Young Lee, Gyeong-Im Choe, Jin-Ju Nah, ByungJae So, Kyung-Woo Lee, Ki-Yoon Chang, Jae-Young Song, Sang-Ho Cha

Animal, Plant and Fisheries Quarantine and Inspection Agency

돼지 지방 조직 및 골수 유래 성체줄기세포의 성상분석과 다능성에 관한 연구

이아영, 최경임, 나진주, 소병재, 이경우, 장기윤, 송재영, 차상호

농림수산검역검사본부

Abstract

Mesenchymal stem cells (MSCs) have ability to differentiate into multi-lineage cells, which confer a great promise for regenerative medicine to the cells. The aim of this study was to establish a method for isolation and characterization of adipose tissue-derived MSC (pAD-MSC) and bone marrow-derived MSC (pBM-MSC) in pigs. Isolated cells from all tissues were positive for CD29, CD44, CD90 and CD105, but negative for hematopoietic stem cell associated markers, CD45. In addition, the cells expressed the transcription factors, such as Oct4, Sox2, and Nanog by RT-PCR. pAD-MSC and pBM-MSC at early passage successfully differentiated into chondrocytes, osteocytes and adipocytes. Collectively, pig AD-MSC and BM-MSC with multipotency were optimized in our study.

Key Words: mesenchymal stem cells, isolation, pig, adipose tissue, bone marrow