Korean J Vet Res > Volume 53(2); 2013 > Article
Korean Journal of Veterinary Research 2013;53(2):95-101.
DOI: https://doi.org/10.14405/kjvr.2013.53.2.095    Published online June 25, 2013.
Comparative evaluation of the murine immune responses to Salmonella enterica serovars Enteritidis, Gallinarum and Typhimurium infection
Kiju Kim1, Dooree Kim1, Jisun Sun1, Soyeon Park1, Youngjae Cho1, Hyun-Jeong Ko2, Hong-Gu Joo3, Tae-Wook Hahn1
1College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University
2College of Pharmacy, Kangwon National University
3College of Veterinary Medicine, Jeju National University
Salmonella enterica serovars Enteritidis, Gallinarum 및 Typhimurium의 마우스 면역반응의 비교평가
김기주1, 김두리1, 선지선1, 박소연1, 조영재1, 고현정2, 주홍구3, 한태욱1
1강원대학교 수의과대학 및 동물의학종합연구소
2강원대학교 약학대학
3제주대학교 수의과대학
The study was carried out to evaluate and compare the immune responses in mice experimentally infected with either wild-type or isogenic mutants of Salmonella enterica serovars Enteritidis (SE), Salmonella Typhimurium (ST) and Gallinarum (SG). The mutant strains were constructed by allelic replacement of some virulence-associated genes in the wild-type strains. Seven-week-old female BALB/c mice were orally or intraperitoneally inoculated by injecting bacterial suspension. To evaluate the immune responses, enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunospot (ELISPOT) assay were conducted with serum and fecal samples. As a result, the mice group infected orally with the SE mutant strain showed the highest level of specific IgA-secreting splenocytes, compared to the other groups. The peritoneally injected groups showed the greater levels of IgG1 than the orally injected groups, which was in a good agreement with the previous studies. In addition, the mutant infected groups had the similar secretion levels of antibodies with the wild-type infected groups. These results demonstrated that the SE mutant strain elicited humoral immune response as much as wild-type, implying that it can be useful as a delivery vehicle as well as a candidate of a live attenuated vaccine.
Key Words: humoral immunity, mucosal immunity, Salmonella

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