Korean J Vet Res > Volume 53(3); 2013 > Article
Korean Journal of Veterinary Research 2013;53(3):143-147.
DOI: https://doi.org/10.14405/kjvr.2013.53.3.143    Published online September 26, 2013.
Induction of apoptosis in mouse spleen cells by Ginsenoside Rp1
Young-Kyun Oh, Hong-Gu Joo
Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University
마우스 비장세포에서 Ginsenoside Rp1의 세포자멸사 유도
오영균, 주홍구
제주대학교 수의과대학 수의약리학실험실
Abstract
Ginsenoside Rp1 is one of ginseng saponins with chemotherapeutic activity. In this study, we investigated the effects of Rp1 on spleen cells. Spleen is a major immune organ consisted of crucial immune cells, such as T lymphocytes, B lymphocytes, natural killer cells, and some antigen-presenting cells. Although the anti-tumor potential of Rp1 was studied, the effects of Rp1 on immune cells have not investigated yet. A viability assay using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), flow cytometric analysis, Western blot analysis were used to detect cellular changes on Rp1-treated spleen cells. MTT assay showed that Rp1 decreased the viability of spleen cells. To further investigate the effects of Rp1 on activated spleen cells, we treated lipopolysaccharide (LPS) as a representative inflammatory agent and Rp1 on spleen cells in a combination. The surface expression levels of activation markers for lymphocytes, CD25 and CD69 were measured. Apoptotic analysis revealed the cytotoxic effects of Rp1 on both na$ddot{i}$ve and activated cells, and the expression pattern of some apoptosis-related proteins was correlated to apoptotic events of cells. Taken together, ginsenoside Rp1 increases the cellular death of spleen cells and also inhibits the LPS-induced activation of spleen cells.
Key Words: apoptosis, cellular toxicity, ginsenoside, Rp1, spleen cells
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