| Induction of apoptosis in mouse spleen cells by Ginsenoside Rp1 |
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Young-Kyun Oh, Hong-Gu Joo |
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Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Jeju National University |
| 마우스 비장세포에서 Ginsenoside Rp1의 세포자멸사 유도 |
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오영균, 주홍구 |
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제주대학교 수의과대학 수의약리학실험실 |
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| Abstract |
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Ginsenoside Rp1 is one of ginseng saponins with chemotherapeutic activity. In this study, we investigated the effects of Rp1 on spleen cells. Spleen is a major immune organ consisted of crucial immune cells, such as T lymphocytes, B lymphocytes, natural killer cells, and some antigen-presenting cells. Although the anti-tumor potential of Rp1 was studied, the effects of Rp1 on immune cells have not investigated yet. A viability assay using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), flow cytometric analysis, Western blot analysis were used to detect cellular changes on Rp1-treated spleen cells. MTT assay showed that Rp1 decreased the viability of spleen cells. To further investigate the effects of Rp1 on activated spleen cells, we treated lipopolysaccharide (LPS) as a representative inflammatory agent and Rp1 on spleen cells in a combination. The surface expression levels of activation markers for lymphocytes, CD25 and CD69 were measured. Apoptotic analysis revealed the cytotoxic effects of Rp1 on both na$ddot{i}$ve and activated cells, and the expression pattern of some apoptosis-related proteins was correlated to apoptotic events of cells. Taken together, ginsenoside Rp1 increases the cellular death of spleen cells and also inhibits the LPS-induced activation of spleen cells. |
| Key Words:
apoptosis, cellular toxicity, ginsenoside, Rp1, spleen cells |
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