| Immunohistochemical localization of galectin-3 in the brain with Theiler's murine encephalomyelitis virus (DA strain) infection |
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Taekyun Shin1, Francisco J. Carrillo-Salinas2, Ana Feliu Martinez2, Miriam Mecha2, Carmen Guaza2 |
1Department of Veterinary Anatomy, College of Veterinary Medicine, Jeju National University
2Department of Functional and Systems Neurobiology, Neuroimmunology Group, Instituto Cajal, CSIC |
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| Abstract |
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Galectin-3 is a ${eta}$-galactoside-binding lectin that plays a role in neuroinflammation through cell migration, proliferation, and apoptosis. In the present study, regulation of galectin-3 was examined in the brain of mice infected with the Daniel strain of Theiler's murine encephalomyelitis virus (TMEV) at days 7 and 81 post-infection by immunohistochemistry. Immunohistochemistry revealed that galectin-3 was mainly localized in ionized calcium-binding adapter 1-positive macrophages/activated microglia, but not in Iba-1-positive ramified microglia. Galectin-3 was also weakly detected in some astrocytes in the same encephalitic lesions, but not in neurons and oligodendrocytes. Collectively, the present findings suggest that galectin-3, mainly produced by activated microglia/macrophages, may be involved in the pathogenesis of virus induced acute inflammation in the early stage as well as the chronic demyelinating lesions in Daniel strain of TMEV induced demyelination model. |
| Key Words:
encephalomyelitis, galectin-3, macrophages, microglia, Theiler's murine encephalomyelitis virus |
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