| Development and evaluation of protective capacity of Salmonella Enteritidis polyphosphate kinase-deleted and temperature-sensitive mutant |
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Kiju Kim1, Soyeon Park1, Youngjae Cho1, Jeong-Yeon Kwak1, Zheng-Wu Kang1, Eun-Hee Kim2, Hwan-Won Choi2, Ho-Keun Won2, Yun-Hee Noh2, Tae-Wook Hahn1 |
1College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University
2ChoongAng Vaccine Lab. |
| Salmonella enterica serovars Enteritidis의 온도감수성 변이주 및 폴리인산키나아제 변이주의 제작과 방어효과 |
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김기주1, 박소연1, 조영재1, 곽정연1, 강정무1, 김은희2, 최환원2, 원호근2, 노윤희2, 한태욱1 |
1강원대학교 수의과대학 및 동물의학종합연구소
2(주)중앙백신연구소 |
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| Abstract |
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This study was focusing on evaluating the protection of polyphosphate kinase (ppk) deleted and/or temperature-sensitive (ts) Salmonella Enteritidis (SE) as an attenuated vaccine in chickens. We constructed SEppk, SEts and SEppk::ts mutants and screened those mutants by growth capability in vitro, protection study in mice model and antibody response in chickens. Among the mutants, SEppk::ts-3 was selected because it showed higher growth capability, good protection against highly virulent SE in mice model, and good antibody response in chickens. SEppk::ts-3 also showed good protection against highly virulent SE isolate because it decreased colonization of virulent SE challenge strain in spleen, liver and cecum compared with the non-vaccinated control. The SEppk::ts-3 mutant showed cross-protection against S. Gallinarum (SG) challenge although the its cross-protection rate was a little lower than that of SG9R, a commercial vaccine against SG infection. To use for live attenuated vaccine in chickens, it should further be characterized. |
| Key Words:
cross protection, polyphosphate kinase deleted mutant, Salmonella Enteritidis, temperature-sensitive mutant |
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