Expression of the S glycoprotein of transmissible gastroenteritis virus (TGEV) in transgenic potato and its immunogenicity in mice |
Dong-Joo Ahn1, Jung Won Youm1, Suk Weon Kim2, Won Kee Yoon3, Hyoung Chin Kim3, Tai-Young Hur4, Young Hee Joung5, Jae-Heung Jeon1, Hyun Soon Kim1 |
1Plant System Engineering Center, KRIBB 2Microbial Resource Center, KRIBB 3Laboratory Animal Resource Center, KRIBB 4National Institute of Animal Science, RDA 5School of Biological Sciences and Technology, Chonnam National University |
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Abstract |
Transgenic plants have been tested as an alternative host for the production and delivery of experimental oral vaccines. Here, we developed transgenic potatoes that express the major antigenic sites A and D of the glycoprotein S from transmissible gastroenteritis coronavirus (TGEV-$S_{0.7}$) under three expression vector systems. The DNA integration and mRNA expression level of the TGEV-$S_{0.7}$ gene were confirmed in transgenic plants by PCR and northern blot analysis. Antigen protein expression in transgenic potato was determined by western blot analysis. Enzyme-linked immunosorbent assay results revealed that based on a dilution series of Escherichia coli-derived antigen, the transgenic line P-2 had TGEV-$S_{0.7}$ protein at levels that were 0.015% of total soluble proteins. We then examined the immunogenicity of potato-derived TGEV-$S_{0.7}$ antigen in mice. Compared with the wild-type potato treated group and synthetic antigen treated group, mice treated with the potato-derived antigen showed significantly higher levels of immunoglobulin (Ig) G and IgA responses. |
Key Words:
oral administration, potato-derived antigen, transmissible gastroenteritis virus, transgenic plant |
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