Korean J Vet Res > Volume 53(4); 2013 > Article
Korean Journal of Veterinary Research 2013;53(4):231-237.
DOI: https://doi.org/10.14405/kjvr.2013.53.4.231    Published online December 24, 2013.
Attraction effect against Lycorma delicatula, antioxidant activity and local irritation test of Ailanthus altissima extract
Seung-Jin Lee, Seung-Chun Park
Laboratory of Clinical Pharmacokinetics and Pharmacodynamics, College of Veterinary Medicine, Kyungpook National University
가죽나무 추출물의 꽃매미 유인효과, 항산화 활성 및 국소자극성시험
이승진, 박승춘
경북대학교 수의과대학 약동력학실험실
Abstract
This study aimed to assess the attraction effect against Lycorma delicatula and antioxidant activity of hexane, chloroform, butanol and water fraction obtained from Ailanthus altissima methanol extract. The attraction effect of chloroform fraction showed the highest activity (47%) as compared to that of other fractions. In the DPPH radical scavenging activity, methanol and butanol fraction showed higher antioxidant activity than other solvent fractions. From the above results, the potential chloroform fraction was further performed by local irritation test in New Zealand white rabbits. In eye irritation test, chloroform fraction showed moderate irritant at high concentration 0.5 g/site/mL, but there was no eye irritation at low concentration (0.05 g/site/mL). In accordance with the Draize evaluation of skin irritation, the primary irritation index was calculated to 3.3 and 0.68 at high (0.5 g/site/mL) and low concentration (0.05 g/site/mL) causing moderate and mild irritation, respectively. On the basis of this study, Ailanthus altissima chloroform fraction could be safely considered to be a candidate of attractant against Lycorma delicatula.
Key Words: Ailanthus altissima, antioxidative activity, attractant, local irritation test, Lycorma delicatula
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