Antibacterial and therapeutic effects of a combination of Sophora flavescens and Glycyrrhiza uralensis Fischer ethanol extracts on mice infected with Streptococcus pyogenes

Yu, Eun-Ah; Cha, Chun-Nam; Park, Eun-Kee; Yoo, Chang-Yeul; Kim, Suk; Lee, Hu-Jang

doi:2014.54.3.189

Korean J Vet Res > Volume 54(3); 2014 > Article

Brief Communication

Korean Journal of Veterinary Research 2014;54(3):189-192.
DOI: https://doi.org/10.14405/kjvr.2014.54.3.189 Published online September 24, 2014.

Antibacterial and therapeutic effects of a combination of Sophora flavescens and Glycyrrhiza uralensis Fischer ethanol extracts on mice infected with Streptococcus pyogenes

Eun-Ah Yu¹, Chun-Nam Cha², Eun-Kee Park³, Chang-Yeul Yoo⁴, Suk Kim⁵, Hu-Jang Lee⁶

¹Tongyeong National Quarantine Station, Ministry of Health & Welfare
²Department of Industrial Systems Engineering, Engineering Research Institute, Gyeongsang National University
³Department of Medical Humanities and Social Medicine, College of Medicine, Kosin University
⁴Department of Computer Information, Gyeongnam Provincial Namhae College
⁵College of Veterinary Medicine, Research Institute of Live Sciences, Gyeongsang National University
⁶Graduate School of Public Health, Gyeongsang National University

Abstract

This study evaluated the antibacterial effects of a mixture of Sophorae radix and Glycyrrhiza uralensis Fischer (1 : 1) ethanol extracts (SGE) on mice infected with Streptococcus (S.) pyogenes. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration of SGE necessary for antibacterial effects against S. pyogenes were $20{mu}g/mL$. Based on the time-kill curves for S. pyogenes, SGE was effective at $4{ imes}$ MIC after 16 h. On Day 12 after challenge, the survival rate of mice treated with 2.0 mg/kg SGE was 60%. In conclusion, SGE had potent in vitro and in vivo antibacterial activities against S. pyogenes.

Key Words: crude extracts, medicinal plants, mice, Streptococcus pyogenes, treatment efficacy