Serological monitoring on brucellosis in livestock of Korea

Sung, So-Ra; Kim, Ji-Yeon; Her, Moon; Lee, Kichan; Kang, Sung-Il; Lee, Hyang-Keun; Cho, Hyo Rim; Lee, Jin Ju; Jung, Suk Chan

doi:2014.54.4.197

Korean J Vet Res > Volume 54(4); 2014 > Article

Original Article

Korean Journal of Veterinary Research 2014;54(4):197-201.
DOI: https://doi.org/10.14405/kjvr.2014.54.4.197 Published online December 22, 2014.

Serological monitoring on brucellosis in livestock of Korea

So-Ra Sung, Ji-Yeon Kim, Moon Her, Kichan Lee, Sung-Il Kang, Hyang-Keun Lee, Hyo Rim Cho, Jin Ju Lee, Suk Chan Jung

OIE Reference Laboratory for Brucellosis, Bacterial Disease Division, Animal and plant Quarantine Agency

국내 가축에서 브루셀라병에 대한 혈청학적 모니터링

성소라, 김지연, 허문, 이기찬, 강성일, 이향근, 조효림, 이진주, 정석찬

농림축산검역본부 세균질병과 브루셀라병 OIE 표준실험실

Abstract

In Korea, brucellosis has been reported periodically in cattle and rarely in dogs; however, it has not previously been screened in domestic animals such as elk, pigs and goats. To investigate the serological prevalence, serum samples were taken from the aforementioned animals annually during 2007-2013 and screened by the rose-bengal test (RBT) or modified RBT, after which positive sera were evaluated by the standard tube agglutination test (STAT). Finally, RBT and STAT-positive sera were confirmed by competitive-ELISA. Brucella abortus biovar 1 was isolated from three elk that were shown to be positive serologically in 2008. There was no evidence of brucellosis in pigs. Based on serological monitoring and investigation of etiological agents, there is no evidence of outbreak of brucellosis in elk, pigs or goats of Korea since 2008. However, the possibility for brucellosis from cattle to affect these other livestock exists; therefore, extensive and continuous serological monitoring is required to maintain their brucellosis-free status.

Key Words: brucellosis, competitive-ELISA, rose-Bengal test, serological test, standard tube agglutination test