Korean J Vet Res > Volume 55(1); 2015 > Article
Korean Journal of Veterinary Research 2015;55(1):9-12.
DOI: https://doi.org/10.14405/kjvr.2015.55.1.9    Published online March 24, 2015.
Comparison of transport media for the isolation and detection of Brachyspira hyodysenteriae
Se-Ji Cho1, Jong Wan Kim1, Ha-Young Kim1, Sang-Ik Oh1, So Jeong Jeong1, Ji-A Jung1, Ara Cho1, Myoung-Heon Lee1, Ho-Seong Cho2, Jae-Won Byun1
1Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency
2College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University
돈적리 균의 분리, 검출을 위한 수송배지의 비교
조세지1, 김종완1, 김하영1, 오상익1, 정소정1, 정지아1, 조아라1, 이명헌1, 조호성2, 변재원1
1농림축산검역본부
2전북대학교 수의과대학
Abstract
Brachyspira (B.) hyodysenteriae is a causative agent of swine dysentery that is responsible for death and economic losses in the pig industry. It is imperative that clinical samples be delivered fresh for accurate diagnosis. The viability and DNA detection of B. hyodysenteriae using lab-made (phosphate buffered saline and modified tryptic soy broth) or commercial transport media (C, D, and E) were compared by culturing and real-time PCR at $4^{circ}C$ or room temperature (RT), respectively. B. hyodysenteriae grown in D (Anaerobe Systems, USA) and E (Starplex Scientific, Canada) media was viable for 4 days at $4^{circ}C$ and RT. However, B. hyodysenteriae in A, B, and C (culture swab; BD Biosciences, USA) media were not recovered after 2 days at RT. Ct values for real-time PCR at $4^{circ}C$ and RT ranged from $27.2{pm}2.1$ (C) to $29.6{pm}0.5$ (B), and $28.0{pm}0.9$ (E) to $30.2{pm}1.5$ (B), respectively. Considering the field conditions, it is important that transport media is used for specimen isolation and PCR to obtain an accurate diagnosis of swine dysentery.
Key Words: Brachyspira hyodysenteriae, pigs, real-time PCR, transport media


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