Korean J Vet Res > Volume 55(2); 2015 > Article
Korean Journal of Veterinary Research 2015;55(2):117-123.
DOI: https://doi.org/10.14405/kjvr.2015.55.2.117    Published online June 24, 2015.
Antibacterial activities of bark extracts from Fraxinus rhynchophylla Hance and Geranium koreanum Kom. against clinical strains of Clostridium perfringens in chickens
Jae-Young Oh1, Kwang-Jae Lee2, Bai Wei1, Jae-Hee Roh1, Min Kang1, Se-Yeoun Cha1, Hyung-Kwan Jang1
1Department of Infectious Diseases and Avian Diseases, College of Veterinary Medicine and Korea Zoonosis Research Institute, Chonbuk National University
2Gangwon Agricultural Research and Extension Services
Necrotic enteritis (NE) caused by Clostridium (C.) perfringens commonly occurs in domestic broiler farms since antibiotic supplementation in poultry feed has been banned. We evaluated the antibacterial activities of medicinal plant extracts against C. perfringens isolates to select alternative compounds for preventing NE. We compared antibacterial activities using two methods and evaluated susceptibilities of the isolates based on minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Two (Fraxinus rhynchophylla Hance [FRH] and Geranium koreanum Kom. [GKK]) of the 30 plant extracts had potent antibacterial activities against C. perfringens ATCC 13124 in two assays. The MIC values for FRH and GKK against 20 C. perfringens isolates were $128{sim}256{mu}g/mL$ and $32{sim}128{mu}g/mL$, respectively. The geometric MIC mean values for the two extracts were $147.2{mu}g/mL$ and $68.8{mu}g/mL$, respectively. The MBCs for the two extracts against the same strains were $1,024{sim}2,048{mu}g/mL$ and $256{sim}1,024{mu}g/mL$, respectively. The geometric mean MIC and MBC for GKK were about two-fold lower than those of FRH. The modified spot-on-lawn assay may be useful for measuring primary antibacterial potential. FRH and GKK are expected to be used as feed additives to prevent or treat NE in veterinary practice.
Key Words: Clostridium perfringens, feed additives, Fraxinus rhynchophylla Hance, Geranium koreanum Kom., necrotic enteritis
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