Korean J Vet Res > Volume 55(3); 2015 > Article
Korean Journal of Veterinary Research 2015;55(3):191-197.
DOI: https://doi.org/10.14405/kjvr.2015.55.3.191    Published online September 22, 2015.
Virulence factors, antimicrobial resistance patterns, and genetic characteristics of hydrogen sulfide-producing Escherichia coli isolated from swine
Hyun-Eui Park, Min-Kyoung Shin, Hong-Tae Park, Seung Won Shin, Myunghwan Jung, Young Bin Im, Han Sang Yoo
Department of Infectious Disease, College of Veterinary Medicine, Seoul National University
Escherichia (E.) coli is commensal bacteria found in the intestine; however, some pathogenic strains cause diseases in animals and humans. Although E. coli does not typically produce hydrogen sulfide ($H_2S$), $H_2S$-producing strains of E. coli have been identified worldwide. The relationship between virulence and $H_2S$ production has not yet been determined. Therefore, characteristics of $H_2S$-producing isolates obtained from swine feces were evaluated including antibiotic resistance patterns, virulence gene expression, and genetic relatedness. Rates of antibiotic resistance of the $H_2S$-producing E. coli varied according to antibiotic. Only the EAST1 gene was detected as a virulence gene in five $H_2S$-producing E. coli strains. Genes conferring $H_2S$ production were not transmissible although the sseA gene encoding 3-mercaptopyruvate sulfurtransferase was detected in all $H_2S$-producing E. coli strains. Sequences of the sseA gene motif CGSVTA around Cys238 were also identical in all $H_2S$- producing E. coli strains. Diverse genetic relatedness among the isolates was observed by pulsed-field gel electrophoresis analysis. These results suggested that $H_2S$-producing E. coli strains were not derived from a specific clone and $H_2S$ production in E. coli is not associated with virulence genes.
Key Words: sseA, antibiotic resistance, Escherichia coli, hydrogen sulfide, virulence factor

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