Korean J Vet Res > Volume 55(3); 2015 > Article
Korean Journal of Veterinary Research 2015;55(3):205-208.
DOI: https://doi.org/10.14405/kjvr.2015.55.3.205    Published online September 22, 2015.
Fecal microbiota analysis of obese dogs with underlying diseases: a pilot study
Hyung Jin Park1, Sang Eun Lee2, Hyeun Bum Kim3, Jae Hoon Kim1, Kyoung Won Seo1, Kun Ho Song1
1College of Veterinary Medicine, Chungnam National University
2Division of Malaria and Parasitic Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention
3Department of Animal Resources Science, Dankook University
Abstract
Ten dogs were enrolled in this study: two healthy dogs, two obese dogs without other medical issues and six obese dogs with underlying diseases including pemphigus, chronic active hepatitis, hyperadrenocorticism, narcolepsy, otitis media and heartworm infection. Pyrosequencing of the 16S rRNA gene to explore the gut bacterial diversity revealed that distal gut bacterial communities of samples from patients with pemphigus, otitis media and narcolepsy consisted primarily of Firmicutes, while the major phylum of the distal gut bacterial communities in patients with chronic active hepatitis and hyperadrenocorticism was Fusobacteria. Proteobacteria were the dominant phylum in heartworm infected obese patients.
Key Words: 16S rRNA, dog, fecal microbiota, obese, pyrosequencing
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