| Anti-thrombotic activity of fermented rice bran extract with several oriental plants in vitro and in vivo |
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Bo-Ra Jeon1, Hyun Dong Ji1, Su Jung Kim2, Chun-Hee Lee3, Tae-Wan Kim1, Man-Hee Rhee1 |
1Laboratory of Veterinary Physiology and Cell Signaling, College of Veterinary Medicine, Kyungpook National University
2Department of Clinical Pathology, Daegu Health College
3Enzyme LAPA Co. |
| 쌀겨발효추출물의 항혈전효과 |
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전보라1, 지현동1, 김수정2, 이천휘3, 김태완1, 이만휘1 |
1경북대학교 수의과대학
2대구보건대학교
3엔자임라파 |
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| Abstract |
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Although the effects of the rice bran have recently been investigated, there is no information regarding platelet physiology available. However, it is well known that fermented natural plants have a beneficial effect on cardiovascular diseases. Therefore, this study was conducted to investigate whether fermented rice bran extract (FRBE) with several plants (Artemisia princeps, Angelica Gigantis Radix, Cnidium officinale, and Camellia sinensis) affected agonist-induced platelet aggregation, and if so, what the underlying mechanism of its activity was. We performed several experiments, including in vitro platelet aggregation, intracellular calcium concentration and adenosine triphosphate release. In addition, the activation of integrin ${alpha}_{II}b{eta}3$ was determined using fibrinogen binding. Thrombus formation was also evaluated in vivo using an arterio-venous shunt model. The FRBE inhibited collagen-induced platelet aggregation in a concentration-dependent manner. FRBE significantly and dose dependently attenuated thrombus formation using rat arterio-venous shunt. FRBE suppressed the intracellular calcium mobilization in collagen-stimulated platelets. We also found that FRBE inhibited extracellular stimuli-responsive kinase 1/2, p38-mitogen-activated protein kinases and c-Jun N-terminal kinase phosphorylation. These results suggested that FRBE inhibited collagen-induced platelet aggregation, which was mediated by modulation of downstream signaling molecules. In conclusion, FRBE could be developed as a functional food against aberrant platelet activation-related cardiovascular diseases. |
| Key Words:
collagen, mitogen-activated protein kinase, platelet aggregation, rice bran, thrombosis |
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