Effect of a mixture of Galla rhois and Cinnamomum cassia extracts on susceptibility to the colonization of Campylobacter jejuni in broiler chickens

Cho, Byung-Wook; Lee, Soo-Mi; Cha, Chun-Nam; Yoo, Chang-Yeol; Son, Song-Ee; Kim, Suk; Lee, Hu-Jang

doi:2016.56.1.9

Korean J Vet Res > Volume 56(1); 2016 > Article

Original Article

Korean Journal of Veterinary Research 2016;56(1):9-14.
DOI: https://doi.org/10.14405/kjvr.2016.56.1.9 Published online May 2, 2016.

Effect of a mixture of Galla rhois and Cinnamomum cassia extracts on susceptibility to the colonization of Campylobacter jejuni in broiler chickens

Byung-Wook Cho¹, Soo-Mi Lee², Chun-Nam Cha³, Chang-Yeol Yoo⁴, Song-Ee Son⁵, Suk Kim⁵, Hu-Jang Lee²

¹Department of Animal Science, College of Life Sciences, Pusan National University
²Department of Environmental Health, Graduate School of Public Health, Gyeongsang National University
³Engineering Research Institute and Department of Industrial Systems Engineering, Gyeongsang National University
⁴Department of Smart Information Convergence, Gyeongnam Provincial Namhae College
⁵Research Institute of Life Sciences and College of Veterinary Medicine, Gyeongsang National University

Abstract

The present study evaluated the effects of a mixture of Galla rhois and Cinnamomum cassia extracts (GCE) (1 : 1, w/w) on susceptibility to the colonization of Campylobacter (C.) jejuni in broilers. Eighty two-week-old broilers (n = 20 per group) were used to estimate the efficacy of GCE against C. jejuni infection via drinking water. Antibacterial activity testing revealed that the minimum bactericidal concentration of GCE against C. jejuni was 2.5 mg/mL. Broilers challenged with C. jejuni were administered 0.0 (Non-GCE), 2.5 (GCE-2.5), 5.0 (GCE-5.0) and 10.0 g/L (GCE-10) GCE for 7 days, and the cecal contents were collected from five broilers per group on the 1st, 3rd, 5th, and 7th day post-treatment. On day 3 post-administration, the number of C. jejuni in GCE-5.0 (p < 0.05) and GCE-10 (p < 0.01) was significantly decreased relative to Non-GCE, while on day 7 those in all GCE-treated groups were significantly decreased compared to the Non-GCE group (p < 0.001). Hematological and blood biochemical analysis revealed no significant differences in parameters between the Non-GCE and GCE-treated groups. Based on the results of the present study, GCE was identified as a safe and alternative candidate to suppress C. jejuni colonization in broilers.

Key Words: Campylobacter jejuni, Cinnamomum cassia, Galla rhois, broiler chicken