Korean J Vet Res > Volume 56(1); 2016 > Article
Korean Journal of Veterinary Research 2016;56(1):45-46.
DOI: https://doi.org/10.14405/kjvr.2016.56.1.45    Published online May 2, 2016.
Isolation and identification of Vibrio harveyi from chub mackerel (Scomber japonicus)
Young-Ran Lee1, Jin-Woo Jun1, Sib Sankar Giri1, Hyoun-Joong Kim1, Sae-Kil Yun1, Cheng Chi1, Sang-Guen Kim1, Jeong-Rack Koh2, Ji-Yun Jung2, Se Chang Park1
1Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University
2Lotteworld Aquarium
Abstract
For several days, there was a series of mortalities of chub mackerel (Scomber japonicus) that were reared for public exhibition in a private aquarium in Seoul, Korea. As part of the diagnosis of the dead fish, a bacterial isolate from the kidney was cultured, identified, and confirmed to be Vibrio (V.) harveyi using Vitek System 2 and 16S rRNA gene sequencing. Phylogenetic analysis was also performed by the neighbor-joining method. As a result, the V. harveyi isolated from chub mackerels of a private aquarium in Korea, called as SNUVh-LW1, was clustered in the same group with V. harveyi ATCC33843.
Key Words: Vibrio harveyi, aquarium, chub mackerel


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