| Evaluation of antibacterial and therapeutic effects of egg-white lysozyme against Salmonella Typhimurium in ICR mice infected with Salmonella Typhimurium |
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Hee-Gyu Kim1, Jae-Seung Hwang2, Woo-Young Jae2, Song-Ee Son3, Hu-Jang Lee3 |
1Uiryeong High School
2Jinju High School
3College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University |
| 난백 유래 라이소자임의 마우스 살모넬라증에 대한 항균 및 치료 효과 |
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김희규1, 황재승2, 제우영2, 손송이3, 이후장3 |
1의령고등학교
2진주고등학교
3경상대학교 수의과대학 |
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| Abstract |
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Salmonellosis is a major bacterial zoonosis that causes self-limited enteritis in animals and foodborne disease and typhoid fever in humans. Recently, multi-drug-resistant strains of Salmonella spp. have increased and caused more serious problems in public health. The present study investigated the antibacterial effects of egg-white lysozyme (EWL) against Salmonella (S.) Typhimurium and the therapeutic effects of EWL for murine salmonellosis. Evaluation of the antibacterial effects of EWL against S. Typhimurium revealed a minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of EWL of 6.25 and $300{mu}g/mL$, respectively. In the bacterial growth inhibition test, EWL at 300 (p < 0.05) and $600{mu}g/mL$ (p < 0.01) significantly inhibited the growth of S. Typhimurium at 4 h post-incubation. EWL administration at MIC (LYS-1), MBC (LYS-2) and $2{ imes}MBC$ (LYS-3) for 14 days resulted in mortality of mice infected with S. Typhimurium of 70, 40 and 10%, respectively, while that of control mice (CON) was 90%. Counts of S. Typhimurium in murine spleens were significantly lower in LYS-2 and LYS-3 than CON (p < 0.05). The results of this study indicate that EWL has the potential for treatment of ICR mice infected with S. Typhimurium. |
| Key Words:
ICR mouse, egg-white lysozyme, salmonellosis, therapeutic effect |
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