Korean J Vet Res > Volume 56(3); 2016 > Article
Korean Journal of Veterinary Research 2016;56(3):189-192.
DOI: https://doi.org/10.14405/kjvr.2016.56.3.189    Published online October 11, 2016.
Correlations in the results of virus neutralization test, hemagglutination inhibition test, and enzyme-linked immunosorbent assay to determine infectious bronchitis virus vaccine potency
Mi-Ja Park1, Seong-Joon Joh1, Kang-Seuk Choi2, Aeran Kim1, Min-Goo Seo1, Jae-Young Song1, Seon-Jong Yun1
1Veterinary Medicine and Biologics Division, Animal and Plant Quarantine Agency
2Avian Diseases Division, Animal and Plant Quarantine Agency
Abstract
The virus neutralization (VN) test was used to determine potency of the infectious bronchitis (IB) vaccine. The results of VN, hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA) were compared with those of the IBV M41. The $r^2$ values between VN and HI titers and the ELISA antibody titer were 0.8782 and 0.0336, respectively, indicating a high correlation between VN and HI, but not VN and ELISA. The Cohen's kappa coefficient between the VN titer of 2 $log_{10}$ and HI titer of 5 $log_2$ was 0.909. Our results showed that VN could be replaced with HI for testing the potency of IBV M41.
Key Words: Massachusetts 41 vaccine, hemagglutination inhibition, infectious bronchitis, potency test
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