Correlations in the results of virus neutralization test, hemagglutination inhibition test, and enzyme-linked immunosorbent assay to determine infectious bronchitis virus vaccine potency

Park, Mi-Ja; Joh, Seong-Joon; Choi, Kang-Seuk; Kim, Aeran; Seo, Min-Goo; Song, Jae-Young; Yun, Seon-Jong

doi:2016.56.3.189

Korean J Vet Res > Volume 56(3); 2016 > Article

Brief Communication

Korean Journal of Veterinary Research 2016;56(3):189-192.
DOI: https://doi.org/10.14405/kjvr.2016.56.3.189 Published online October 11, 2016.

Correlations in the results of virus neutralization test, hemagglutination inhibition test, and enzyme-linked immunosorbent assay to determine infectious bronchitis virus vaccine potency

Mi-Ja Park¹, Seong-Joon Joh¹, Kang-Seuk Choi², Aeran Kim¹, Min-Goo Seo¹, Jae-Young Song¹, Seon-Jong Yun¹

¹Veterinary Medicine and Biologics Division, Animal and Plant Quarantine Agency
²Avian Diseases Division, Animal and Plant Quarantine Agency

Abstract

The virus neutralization (VN) test was used to determine potency of the infectious bronchitis (IB) vaccine. The results of VN, hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA) were compared with those of the IBV M41. The $r^2$ values between VN and HI titers and the ELISA antibody titer were 0.8782 and 0.0336, respectively, indicating a high correlation between VN and HI, but not VN and ELISA. The Cohen's kappa coefficient between the VN titer of 2 $log_{10}$ and HI titer of 5 $log_2$ was 0.909. Our results showed that VN could be replaced with HI for testing the potency of IBV M41.

Key Words: Massachusetts 41 vaccine, hemagglutination inhibition, infectious bronchitis, potency test