| Analysis of antibiotic residues in milk from healthy dairy cows treated with bovine mastitis ointment using ultra-performance liquid chromatography coupled with electrospray tandem mass spectrometry |
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Eun-Kee Park1, Yong-Jae Ryu2, Chun-Nam Cha3, Chang-Yeul Yoo4, Suk Kim5, Hu-Jang Lee5 |
1Medical Humanities and Social Medicine, College of Medicine, Kosin University
2Food and Drug Analysis Section, Chungcheongbuk-do Institute of Health and Environment
3Engineering Research Institute and Department of Industrial Systems Engineering, Gyeongsang National University
4Department of Smart Information Convergence, Gyeongnam Provincial Namhae College
5Institute of Animal Medicine and College of Veterinary Medicine, Gyeongsang National University |
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| Abstract |
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This study was conducted to analyze penicillin G (PEG), streptomycin (STR) and neomycin (NEO) residues in milk of healthy lactating cows. Milk samples were collected from all four quarters of 12 dairy cows 2?7 days after intramammary infusions of an ointment containing PEG, STR and NEO once (n = 4; group I) or twice (n = 4, group II) daily. Ultra-performance liquid chromatography coupled with electrospray tandem mass spectrometry was used to determine the antibiotic residues in the samples. The correlation coefficient ($r^2$) of the calibration curves for all antibiotics was > 0.999 and the limits of detection and quantification were $0.002-0.005{mu}g/mL$ and $0.007-0.02{mu}g/mL$, respectively. Recovery rates were ranged from 75.5 to 92.3%. In group I, PEG, STR and NEO residues were detected in milk at 2, 3 and 2 days post-treatment, respectively, which were below the maximum residue limit (MRL). In group II, PEG, STR and NEO residues were detected in milk at 2, 3 and 3 days post-treatment, respectively, which were bellow the MRL. These results suggest that a 3-day for milk withdrawal period after the ointment treatment might be sufficient for reduction of the antibiotic residues below the MRL. |
| Key Words:
UPLC-MS/MS, bovine milk, neomycin, penicillin G, streptomycin |
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