Korean J Vet Res > Volume 57(1); 2017 > Article
Korean Journal of Veterinary Research 2017;57(1):37-42.
DOI: https://doi.org/10.14405/kjvr.2017.57.1.37    Published online April 27, 2017.
Establishment of reverse transcription polymerase chain reaction for detection of Getah virus infection in livestock
Seung Heon Lee, Dong-Kun Yang, Ha-Hyun Kim, Sung-Suk Choi, In-Soo Cho
Viral Disease Division, Animal and Plant Quarantine Agency
Abstract
Getah virus (GETV) infection causes sporadic outbreaks of mild febrile illness in horses and reproductive failure in pigs. In this study, we established a reverse transcription polymerase chain reaction (RT-PCR) method to detect GETV from suspected virus-infected samples. The reaction conditions were optimized and validated by using RNA extracted from GETV propagated in cell culture. A GETV-specific GED4 primer set was designed and used to amplify a 177 bp DNA fragment from a highly conserved region of the E1 glycoprotein gene in the GETV genome. RT-PCR performed with this primer set revealed high sensitivity and specificity. In the sensitivity test, the GED4 primer set detected GETV RNA at the level of $10^{2.0};TCID_{50}/mL$. In the specificity test, the GED4 primer set amplified only a single band of PCR product on the GETV RNA template, without non-specific amplification, and exhibited no cross-reactivity with other viral RNAs. These results suggest that this newly established RT-PCR method is useful for accurate identification of GETV infection in animals.
Key Words: Getah virus, diagnosis, reverse transcription polymerase chain reaction


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