Korean J Vet Res > Volume 57(2); 2017 > Article
Korean Journal of Veterinary Research 2017;57(2):117-126.
DOI: https://doi.org/10.14405/kjvr.2017.57.2.117    Published online August 11, 2017.
Identification of microbiome with 16S rRNA gene pyrosequencing and antimicrobial effect of egg white in bovine mastitis
Danil Kim1, Eun-Kyung Kim1, Won-Jin Seong1, Younghye Ro1, Dae-Sung Ko1, Nam-Hyung Kim2, Jae-Hong Kim2, Hyuk-Joon Kwon1
1Department of Farm Animal Medicine, College of Veterinary Medicine, Seoul National University
2Laboratory of Avian Diseases, College of Veterinary Medicine, Seoul National University
젖소 유방염에서 16S rRNA 파이로시퀀싱을 이용한 우유 내 마이크로바이옴의 동정과 난백의 항균효과
김단일1, 김은경1, 성원진1, 노영혜1, 고대성1, 김남형2, 김재홍2, 권혁준1
1서울대학교 수의과대학 산업동물의학연구실
2서울대학교 수의과대학 조류질병학연구실
Bovine mastitis is an important microbial disease in the dairy industry. We investigated the frequencies of bacterial pathogens in 62 farms and pathogen antibiotic resistance from mastitis samples (n = 748). We tested the antimicrobial activity of chicken and duck egg white and lysozyme purified from chicken egg white. Moreover, we compared the microbiomes of normal and mastitic raw milk obtained by 16S rRNA gene pyrosequencing and culture methods. The results showed that the frequencies of Gram-positive pathogens (Enterococcus faecalis 37% and Staphylococcus aureus 36%) were higher than that of a Gram-negative pathogen (Escherichia coli 15%). Resistance frequencies to ampicillin and norfloxacin were lowest in Staphylococcus aureus (21%), Enterococcus faecalis (23%), and Escherichia coli (33%), and the antimicrobial activity of chicken egg white was higher than those of lysozyme and duck egg white. Pyrosequencing results revealed clear differences between the microbiomes of mastitic and normal raw milk samples and revealed a slightly similar, but clearly different, composition of pathogens compared to that from the culture method. Thus, pyrosequencing may be useful for elucidating changes in microbiomes during mastitis progression and treatment. A chicken egg white and antibiotic combination may help with mastitis treatment; however, further studies are needed.
Key Words: antibiotic resistance, bovine mastitis, microbial pathogens, microbiome, raw milk

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