Korean J Vet Res > Volume 58(1); 2018 > Article
Korean Journal of Veterinary Research 2018;58(1):9-16.
DOI: https://doi.org/10.14405/kjvr.2018.58.1.9    Published online May 8, 2018.
Preliminary assessment of correlation between T-lymphocyte responses and control of porcine reproductive and respiratory syndrome virus (PRRSV) in piglets born after in-utero infection of a type 2 PRRSV
Sang-Ho Cha1, Carey Bandaranayaka-Mudiyanselage2, Chandima B. Bandaranayaka-Mudiyanselage2, Dharani Ajiththos3, Kyoung-Jin Yoon4, Kathleen A. Gibson4, Ji-Eun Yu1, In-Soo Cho1, Stephen S. Lee5, Chungwon J. Chung2
1Animal and Plant Quarantine Agency
2VMRD Inc.
3Department of Veterinary Microbiology and Pathology, Washington State University
4Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University
5University of Idaho
A preliminary study into the protective mechanisms of adaptive immunity against porcine reproductive and respiratory syndrome virus (PRRSV) in piglets (n = 9) born to a gilt challenged intranasally with a type-2 PRRSV. Immune parameters (neutralizing antibodies, $CD3^+CD4^+$, $CD3^+CD8^+$, $CD3^+CD4^+CD8^+$ T-lymphocytes, and PRRSV-specific interferon $(IFN)-{gamma}$ secreting T-lymphocytes) were compared with infection parameters (macro- and microscopic lung lesion, and PRRSV-infected porcine alveolar macrophages ($CD172{alpha}^+PRRSV-N^+;PAM$) as well as with plasma and lymphoid tissue viral loads. Percentages of three T-lymphocyte phenotypes in 14-days post-birth (dpb) peripheral blood mononuclear cell (PBMC) had significant negative correlations with percentages of $CD172{alpha}^+PRRSV-N^+;PAM$ (p < 0.05) as well as with macroscopic lung lesion (p < 0.01). Plasma and tissue viral loads had significant (p < 0.05) negative correlations with $CD3^+CD4^+CD8^+$ T-lymphocyte percentage in PBMC. Frequencies of $CD3^+CD8^+$ and $CD3^+CD4^+$ T-lymphocytes in 14-dpb PBMC had significant negative correlations with of lymph node (p = 0.04) and lung (p = 0.002) viral loads. $IFN-{gamma}$-secreting T-lymphocytes frequency had a significant negative correlation with gross lung lesion severity (p = 0.002). However, neutralizing antibody titers had no significant negative correlation (p > 0.1) with infection parameters. The results indicate that T-lymphocytes contribute to controlling PRRSV replication in young piglets born after in-utero infection.
Key Words: T-lymphocyte, neutralizing antibodies, porcine reproductive and respiratory syndrome virus

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