Korean J Vet Res > Volume 58(1); 2018 > Article
Korean Journal of Veterinary Research 2018;58(1):51-55.
DOI: https://doi.org/10.14405/kjvr.2018.58.1.51    Published online May 8, 2018.
Molecular identification of coagulase-negative staphylococci by rpoB sequence typing
Won-Jin Seong1, Danil Kim1, Eun-Kyung Kim1, Dae-Sung Ko1, Younghye Ro1, Jae-Hong Kim2, Hyuk-Joon Kwon1
1Department of Farm Animal Medicine, College of Veterinary Medicine, Seoul National University
2Laboratory of Avian Diseases, College of Veterinary Medicine, Seoul National University
rpoB 염기서열 분석을 이용한 응고효소 음성 포도알세균 분자 동정
성원진1, 김단일1, 김은경1, 고대성1, 노영혜1, 김재홍2, 권혁준1
1서울대학교 수의과대학 산업동물의학연구실
2서울대학교 수의과대학 조류질병학연구실
Abstract
Bovine mastitis (BM) has resulted in enormous economic loss in the dairy industry and coagulase-negative staphylococci (CNS) have caused subclinical BM. Although VITEK 2 GP ID card (VITEK 2) has been used for CNS identification, the probability of identification varies. The rpoB sequence typing (RSTing) method has been used for molecular diagnosis and epidemiology of bacterial infections. In this study, we undertook RSTing of CNS and compared the results with those of VITEK2 and 16S rRNA gene sequencing. As compared VITEK2, the molecular-based methods were more reliable for species identification; moreover, RSTing provided more molecular epidemiological information than that from 16S rRNA gene sequencing.
Key Words: 16S rRNA gene sequencing, bovine mastitis, coagulase-negative staphylococci, rpoB sequence typing
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