Different invasion efficiencies of Brucella abortus wild-type and mutants in RAW 264.7 and THP-1 phagocytic cells and HeLa non-phagocytic cells

Shim, Soojin; Im, Young Bin; Jung, Myunghwan; Park, Woo Bin; Yoo, Han Sang

doi:2018.58.2.95

Korean J Vet Res > Volume 58(2); 2018 > Article

Brief Communication

Korean Journal of Veterinary Research 2018;58(2):95-98.
DOI: https://doi.org/10.14405/kjvr.2018.58.2.95 Published online July 2, 2018.

Different invasion efficiencies of Brucella abortus wild-type and mutants in RAW 264.7 and THP-1 phagocytic cells and HeLa non-phagocytic cells

Soojin Shim¹, Young Bin Im¹, Myunghwan Jung², Woo Bin Park¹, Han Sang Yoo¹

¹Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University
²Department of Microbiology, Research Institute of Life Sciences, Gyeongsang National University School of Medicine

Abstract

Brucellosis is one of the common zoonoses caused by Brucella abortus (B. abortus). However, little has been reported on factors affecting invasion of B. abortus into host cells. To investigate cell-type dependent invasion of B. abortus, phagocytic RAW 264.7 and THP-1 cells and non-phagocytic HeLa cells were infected with wild-type and mutant B. abortus, and their invasion efficiencies were compared. The invasion efficiencies of the strains were cell-type dependent. Wild-type B. abortus invasion efficiency was greater in phagocytic cells than in epithelial cells. The results also indicated that there are different factors involved in the invasion of B. abortus into phagocytic cells.

Key Words: Brucella abortus, cell invasion, mutants, non-phagocytes, phagocytes