Korean J Vet Res > Volume 58(4); 2018 > Article
Korean Journal of Veterinary Research 2018;58(4):211-217.
DOI: https://doi.org/10.14405/kjvr.2018.58.4.211    Published online February 1, 2019.
Differentiation potential of canine mesenchymal stem cells on hydrogel scaffold-based three-dimensional environment
Na-Yeon Gu, Mi Jeong Park, Jienny Lee, Jeong Su Byeon, Da-Un Jeong, In-Soo Cho, Sang-Ho Cha
Viral Disease Research Division, Animal and Plant Quarantine Agency
하이드로젤 지지체 기반 3차원 환경에서 개 간엽줄기세포의 분화능 분석
구나연, 박미정, 이지현, 변정수, 정다운, 조인수, 차상호
농림축산검역본부 바이러스질병과
Abstract
Mesenchymal stem cells (MSCs) are useful candidates for tissue engineering and cell therapy. Physiological cell environment not only connects cells to each other, but also connects cells to the extracellular matrix that provide mechanical support, thus exposing the entire cell surface and activating signaling pathways. Hydrogel is a polymeric material that swells in water and maintains a distinct 3-dimensional (3D) network structure by cross linking. In this study, we investigated the optimized cellular function for canine adipose tissue-derived MSCs (cAD-MSCs) using hydrogel. We observed that the expression levels of Ki67 and proliferating cell nuclear antigen, which are involved in cell proliferation and stemness, were increased in transwell-hydrogel (3D-TN) compared to the transwell-normal (TN). Also, transforming growth factor-${eta}1$ and SOX9, which are typical bone morphogenesis-inducing factors, were increased in 3D-TN compared to the TN. Collagen type II alpha 1, which is a chondrocyte-specific marker, was increased in 3D-TN compared to the TN. Osteocalcin, which is a osteocyte-specific marker, was increased in 3D-TN compared to the TN. Collectively, preconditioning cAD-MSCs via 3D culture systems can enhance inherent secretory properties that may improve the potency and efficacy of MSCs-based therapies for bone regeneration process.
Key Words: chondrogenesis, extracellular matrix, hydrogels, mesenchymal stem cells, osteogenesis


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