Korean J Vet Res > Volume 59(1); 2019 > Article
Korean Journal of Veterinary Research 2019;59(1):43-45.
DOI: https://doi.org/10.14405/kjvr.2019.59.1.43    Published online May 31, 2019.
Conditions for the disinfectant efficacy test under subzero temperatures
Won-Seok Chae1, Wooseog Jeong2, Hu-Jang Lee3
1Division of Life-Science & Chemistry, Daejin University
2Animal and Plant Quarantine Agency
3Institute of Animal Medicine and College of Veterinary Medicine, Gyeongsang National University
Correspondence:  Hu-Jang Lee, Tel: 82-55-772-2352, Fax: 82-55-772-2308, 
Email: hujang@gnu.ac.kr
To establish appropriate conditions for a disinfectant efficacy test at subzero temperatures, this study examined mixtures of frozen foot-and-mouth disease virus or avian influenza virus solutions and disinfectant diluents at $-5^{circ}C$ and monitored temperature and freezing status of an anti-freezing diluent (AFD, 15% ethanol + 30% propylene glycol + 55% distilled water) over time at various subzero temperatures. Viral solutions and disinfectant diluents froze before the mixtures reached $-5^{circ}C$, whereas the AFD was not frozen at $-30^{circ}C$. The times taken for the AFD to reach -10, -20, -30, and $-40^{circ}C$ from room temperature were 36, 39, 45, and 48 min, respectively.
Key Words: foot-and-mouth disease virus, avian influenza virus, disinfectant efficacy test, subzero temperature, anti-freezing diluent
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