Conditions for the disinfectant efficacy test under subzero temperatures

Chae, Won-Seok; Jeong, Wooseog; Lee, Hu-Jang

doi:2019.59.1.43

Korean J Vet Res > Volume 59(1); 2019 > Article

Brief Communication

Korean Journal of Veterinary Research 2019;59(1):43-45.
DOI: https://doi.org/10.14405/kjvr.2019.59.1.43 Published online May 31, 2019.

Conditions for the disinfectant efficacy test under subzero temperatures

Won-Seok Chae¹, Wooseog Jeong², Hu-Jang Lee³

¹Division of Life-Science & Chemistry, Daejin University
²Animal and Plant Quarantine Agency
³Institute of Animal Medicine and College of Veterinary Medicine, Gyeongsang National University

Correspondence: Hu-Jang Lee, Tel: 82-55-772-2352, Fax: 82-55-772-2308,
Email: hujang@gnu.ac.kr

Abstract

To establish appropriate conditions for a disinfectant efficacy test at subzero temperatures, this study examined mixtures of frozen foot-and-mouth disease virus or avian influenza virus solutions and disinfectant diluents at $-5^{circ}C$ and monitored temperature and freezing status of an anti-freezing diluent (AFD, 15% ethanol + 30% propylene glycol + 55% distilled water) over time at various subzero temperatures. Viral solutions and disinfectant diluents froze before the mixtures reached $-5^{circ}C$, whereas the AFD was not frozen at $-30^{circ}C$. The times taken for the AFD to reach -10, -20, -30, and $-40^{circ}C$ from room temperature were 36, 39, 45, and 48 min, respectively.

Key Words: foot-and-mouth disease virus, avian influenza virus, disinfectant efficacy test, subzero temperature, anti-freezing diluent