| Various macromolecules in in vitro growth medium influence growth, maturation, and parthenogenetic development of pig oocytes derived from small antral follicles |
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Hanna Lee1, Yongjin Lee1, Joohyeong Lee2, Geun-Shik Lee1, Seung Tae Lee3, Eunsong Lee1 |
1College of Veterinary Medicine, Kangwon National University
2Institute of Veterinary Science, Kangwon National University
3Division of Applied Animal Science, Kangwon National University |
| 돼지에서 난자의 체외발육 배양액 내 첨가된 거대분자물질이 작은 난포 유래 미성숙 난자의 성장, 성숙 및 배 발육에 미치는 영향 |
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이한나1, 이용진1, 이주형2, 이근식1, 이승태3, 이은송1 |
1강원대학교 수의과대학
2강원대학교 동물의학종합연구소
3강원대학교 동물생명과학대학 |
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| Abstract |
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This study was performed to examine the effects of various macromolecules in in vitro growth (IVG) media on the growth, maturation, and parthenogenesis (PA) of pig oocytes derived from small antral follicles (SAF). Immature oocytes were cultured for two days in IVG medium supplemented with 10% (v/v) fetal bovine serum (FBS), 10% (v/v) pig follicular fluid (PFF), 0.4% (w/v) bovine serum albumin (BSA), or 0.1% (w/v) polyvinyl alcohol (PVA) and then maintained for 44 h for maturation. After IVG, the mean diameters of the SAF treated with FBS, PVA, and no IVG-MAF ($113.0-114.8{mu}m$) were significantly larger than that of no IVG-SAF ($111.8{mu}m$). The proportion of metaphase II oocytes was higher in PFF (73.6%) than in BSA (43.5%) and PVA (53.7%) but similar to that in the FBS treatment (61.5%). FBS and PFF increased cumulus expansion significantly compared to PVA and BSA while the intraoocyte glutathione content was not influenced by the macromolecules. Blastocyst formation of PA oocytes treated with FBS (51.8%), PFF (50.4%), and PVA (45.2%) was significantly higher than that of the BSA-treated oocytes (20.6%). These results show that the PFF and FBS treatments during IVG improved the growth, maturation, and embryonic development of SAF. |
| Key Words:
in vitro maturation, macromolecules, oocyte growth, small antral follicles |
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