Korean J Vet Res > Volume 59(4); 2019 > Article
Korean Journal of Veterinary Research 2019;59(4):195-199.
DOI: https://doi.org/10.14405/kjvr.2019.59.4.195    Published online February 17, 2020.
Anti-tumor activity and mitochondrial stability of disulfiram in HL-60 cells
Hyowon Shin, Yong Han, Hong-Gu Joo
Veterinary Medical Research Institute, College of Veterinary Medicine, Jeju National University
HL-60세포에서 disulfiram의 항암작용과 미토콘드리아 안정성에 대한 연구
신효원, 한용, 주홍구
제주대학교 수의과대학 수의과학연구소
Correspondence:  Hong-Gu Joo, Tel: 82-64-754-3379, Fax: 82-64-756-3354, 
Email: jooh@jejunu.ac.kr
Abstract
Disulfiram (DSF) is a member of the dithiocarbamate family that can bind copper. Recent studies have shown that DSF has anti-cancer activities, but the mechanism has not been clarified. Therefore, it is important to study the action mechanism of DSF to maximize its anticancer effects. A human leukemia cell line, HL-60, was used in this study. HL-60 cells were treated with DSF and the cellular metabolic activity was measured. DSF increased the cell death of HL-60 cells in annexin V-fluorescein isothiocyanate/propidium iodide staining analysis. In addition, DSF decreased the mitochondrial membrane potential (MMP) of the HL-60 cells. The cytotoxicity of DSF on HL-60 cells was observed at 0.4 μM. Interestingly, the reduction of MMP by DSF was recovered by N-acetyl-L-cysteine, an inhibitor of reactive oxygen species (ROS) production. This suggests that the decrease in MMP by DSF is closely related to the production of ROS in HL-60 cells, which indicates the relationship between the apoptosis of HL-60 cells by DSF and the role of the mitochondria. This study provides clinicians and researchers with valuable information regarding the anti-cancer activity of DSF in terms of the action mechanism.
Key Words: disulfiram, HL-60 cells, mitochondrial membrane potential, cell death, reactive oxygen species
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