Studies on the Methods for the Isolation of salmonella from Pigs

Chung, G.T.

Korean Journal of Veterinary Research 1969;9(1):63-69.

G.T. Chung

Department of Veterinary Medicine, College of Agriculture, Seoul National University

돼지에서 살모넬라균(菌)을 분리(分離)하는 방법(方法)에 관한 연구(硏究)

정길택

서울대학교 농과대학 수의학과

Abstract

Various methods for the isolation of salmonellae were compared by examining mesenteric lymph nodes and faeces of 395 pigs at slaughter. The conclusions were as follows. (i) Tetrathionate broth was significantly superior to selenite broth for the examination of mesenteric lymph nodes. (ii) There was no significant difference between tetrathionate broth and selenite broth for the examination of faeces. The use of both tetrathionate broth and selenite broth as enrichment media for faeces produced far better results than the use of any single enrichment medium. (iii) Brilliant green agar was significantly superior to SS agar for the examination of mesenteric lymph nodes. (iv) There was no significant difference between brilliant green agar and SS agar when faeces were examined. The use of both media produced far better results than the use of either singly. (v) Brilliant green MacConkey broth was much inferior to tetrathionate broth as an enrichment broth. Direct culture of faeces on brilliant green MacConkey agar yielded less isolates than prior enrichment in tetrathionate or selenite broth. (vi) The optimum incubation period of enrichment was 24 hours for faeces and 48 hours for mesenteric lymph nodes when either tetrathionate broth or selenite broth was used as enrichment media.