Studies on the Eurytrema pancreaticum

Jang, Du Hwan

Korean Journal of Veterinary Research 1970;10(1):25-31.

Du Hwan Jang

Department of Veterinary Medicine, College of agriculture, Seoul National University

췌질(膵蛭)에 관한 연구(硏究) [III] 충란검사법(蟲卵檢査法)의 개량(改良)

장두환

서울대학교 농과대학

Abstract

The techniques which have been used for the fecal examination of ruminant infected with the pancreatic flukes, Eurytrema Pancreaticum, were reviewer in their efficiency to detect the ova. One of modified fecal examination: H.F.E. (hydrochloric acid-formalin-ether) sedimentation method was devised in this study. Efficiency in the detecting ability of the fluke eggs with H.F.E. sedimentation method was determined by a series of repeat tests. Among 20 head of cattle known to harbor 1-5 adult worms of the pancreatic fluke, 75% of the infected cattle were detected, and among 60 head of cattle known to harbor more than 6 adult worms, 95% of the infected cattle were detected with H.F.E. sedimentation method. The procedures of the H.F.E. sedimentation method are as follows; 1) Take the sample 5-10 gm., emulsify throughly with 20 ml. of 50% hydrochloric acid in a cup. 2) Strain this mixture through one or two layers of wet surgical gauze into 15ml. centrifuge tube. 3) Washing the cup with 5ml. of 50% hydrochloric acid and strain again. 4) Centrifuge at 2,300 rpm. for 2 minutes. 5) Pour off the supernatant fluid. 6) After the sediment mixed with 10% formalin, stand for 5 minutes. 7) Add 2-3ml. of ether, shake vigorously up and down, after the top of the tube covered with thumb. 8) Centrifuge at 2,300 rpm. for 2 minutes. 9) Loosen the fecal plug in the tube by ringing with an applicator stick. 10) Quickly, but carefully, pour of all, but the bottom layer of sediment. 11) Thoroughly mix the sediment, pour on a slide (or pick up it with a pipett), mount with a cover glass. 12) Examine carefully.