Studies on Separation, Detection and Quantitation of Estriol, Estrone, Estradiol-17 β in Urine of Dairy Cows by Paper, Thin Layer and Column Chromatography

Yang, Yong Kwan; Han, Soo Nam; Cho, Jong Hoo

Korean Journal of Veterinary Research 1973;13(1):23-30.

Yong Kwan Yang¹, Soo Nam Han¹, Jong Hoo Cho²

¹Department of Veterinary Medicine, College of Agriculture, Seoul National University
²Institute of Veterinary Research, Office of Rural Development

Paper, Thin Layer 및 Column Chromatography에 의한 요중의 Estriol, Estrone, Estadiol-17 β의 분리 정량에 관하여

양용관¹, 한수남¹, 조종후²

¹서울대학교 농과대학 수의학과
²농촌진흥청 가축위생연구소

Abstract

Thin layer, paper and column chromatography were compared for the separation, detection and quantitation of three kinds of estrogen in urine of dairy cows. While thin layer chromatography utilizing silica gel was better for the detection of estrogens, column chromatography using celite 545 was preferable. Spectrophotometry was compared with fluorometry for determination of estrone, estradiol-17 ${eta}$ and estriol eluted by paper chromatography and column chromatography. Optical density of three standard estrogens showed almost same curve at maximum absorption wave length of 230 and $282m{mu}$. However, the former showed a higher peak. In fluorometry, the fluorescence intensity of estrone and estradiol-17 ${eta}$ were rather strong, when the estrogens were dissolved in sulfuric acid, and showed higher sensitivity than that of the spectrophotometry. However, in the case of estriol was exceptional.