Studies on the Constituents of Bordetella bronchiseptica. Purification and Properties of the Fractions Obtained by DEAE-cellulose Column Chromatography

Kang, Byong Kyu

Korean Journal of Veterinary Research 1973;13(1):47-52.

Byong Kyu Kang

Department of Veterinary Medicine, College of Agriculture, Jeonnam National University

Bordetella bronchiseptica의 균체성분(菌體成分)에 관한 연구(硏究) 특(特)히 DEAE-cellulose Chromatography에 의한 분획정제(分劃精制)에 대하여

강병규

전남대학교 농과대학 수의학과

Abstract

An attempt was carried out to purify the cell components of Bordetella bronchiseptica by the mild procedures. From the supernatant of sonic treated colls, the K-agglutinogen, hemagglutinogen and skin necrotizing factor were separated by a successive use of DEAE-cellulose column chromatography and ammonium sulfate precipitation. The specific activity of purified K-agglutinogen was demonstrated by a gel-diffusion test and was also found to be free from other biologically active substances of B. bronchiseptica: namely hemagglutinin, skin-necrotizing factor and O-antigen.