Blood Typing of Asian Formosan Deer by Immunological Methods

Lim, Young-Jae; Suzuki, Shozo

Korean Journal of Veterinary Research 1983;23(1):37-44.

Young-Jae Lim¹, Shozo Suzuki²

¹Yong-in Farm Land
²Tokyo University of Agriculture

꽃사슴의 혈액형(血液型)에 관한 연구(硏究) I. 면역반응(免疫反應)에 의한 꽃사슴의 혈액형분류(血液型分類)(제일보(第一報))

임영재¹, 스즈키쇼조²

¹용인자연농원
²동경농업대학

Abstract

The present study was conducted to clarify the presence or absence of isohemagglutinin and isohemolysin and, if present, to classify blood types in the Formosan deer. It was found that, though the titers were relatively low, isohemagglutinin and isohemolysin were present in the deer sera. Results obtained before and after heat treatment of the deer sera at $56^{circ}C$ for 30 minutes were variable, some stable and some unstable to the treatment and still some showing the activities only after the heat treatment. Rabbits, when immunized with deer blood cells, responded with very high titers both in agglutination and hemolysis tests and the activities were not inactivated by the treatment of the immune sera at $56^{circ}C$ for 30 minutes. Using four rabbit immune sera to different deer blood cells following blood types were recognized among 30 heads of deer; 1. by hemagglutination test A : positive to all four immune sere (13 heads). B : positive to the immune sera of 7BS and 8BS but negative to those of 1BS and 3BS (8 heads). C : positive to the sera of 3BS, 7BS and 8BS but negative to that of 1BS (9 heads). 2. by hemolysis test A : positive to all four immune sera (24 heads). B : positive to the immune sera of 7BS and 8BS but negative to those of 1BS and 3BS (3 heads). C : positive to the sera of 3BS, 7BS and 8BS but negative to that of 1BS (3 heads).