| Isolation and Identification of Mycobactin Dependent Acid-fast Bacteria (M. paratuberculosis) from Bovine Fecal Material |
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Yun-seong Jeon1, Bang-whan Lee2, Jong-bae Kim1, Chul-soon Choi3, Jin-koo Kim4 |
1College of Veterinary Medicine, Seoul National University
2Department of Veterinary Medicine, College of Agriculture, Chonnam National University
3College of Medicine, Chung-Ang University
4Green Cross Veterinary Products Co., Ltd. |
| 우유래(牛由來) Mycobactin의존성(依存性) 항산성세균(抗酸性細菌) (M. paratuberculosis)의 분리동정(分離同定) |
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전윤성1, 이방환2, 김종배1, 최철순3, 김진구4 |
1서울대학교 수의과대학
2전남대학교 농과대학 수의학과
3중앙대학교 의과대학
4녹십자수의약품주식회사 |
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| Abstract |
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Fecal material from cattle, which was confirmed to be infected with Johne's disease by clinical and pathological symptoms, was decontaminated with 4% NaOH and inoculated into the $L{ddot{o}}wenstein$-Jensen media supplemented with 1% of heat-killed Mycobacterium bovis. After 2-4 week-incubation at $37^{circ}C$, typical acid-fast mycobacteria was isolated. With the results of staining properties, morphological characteristics, the requirement of mycobactin for growth and the other biochemical properties, isolated mycobacteria was identified as Mycobacterium paratuberculosis. Female guinea pigs were sensitized with the isolates, and skin test was done with purified protein derivatives (PPDs) of M. avium, M. bovis and M. paratuberculosis 4 weeks after sensitization. Animals showed the largest reaction to the PPDs of M. avium and M. paratuverculosis. |
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