| A Study on Antiestrogenic Effects of Tamoxifen in Immature Rat Uterus; I. Effects on Concentrations of Cytosol and Nuclear Estradiol Receptor |
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Hyo-jong Lee1, Choong-ho Jo2, Moo-hyun Park1 |
1Department af Veterinary Medicine, Gyeongsang National University
2College of Veterinary, Medicine, Seoul National University |
| 미성숙 쥐 자궁에서 Tamoxifen의 Antiestrogen 효과에 관한 연구 : I. 세포질 내 및 핵 내 Estradiol 수용체 농도의 변화에 관하여 |
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이효종1, 조충호2, 박무현1 |
1경상대학교 농과대학 수의학과
2서울대학교 수의과대학 |
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| Abstract |
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The Present study has been carried out to elucidate the antiestrogenic effects of tamoxifen in uteri of immature rats. Immature female Sprague-Dawley rats were allocated into 4, groups and injected with $5{mu}g$ of estradiol-$17{eta}$, $50{mu}g$ of tamoxifen, a combination of both or vehicle only subcutaneously three times after an interval of 24 hours respectively. The concentrations, of cytosol estradiol receptor in uterus were measured by DCC method before and 1, 3, 6, 12, 24, 48 and 72 hours after the above treatments and those of nuclear estradiol were measured by protamine exchange method 72 hours and those of nuclear estradiol were measured by protamine exchange method 72 hours after the above treatments. The results obtained were summarized as follows: 1. The binding affinity of tamoxifen to estradiol receptor in uterine cytosol was lower than that of estradiol-$17{eta}$, accordingly the translocation of estradiol receptor into the nucleus was found to be delayed. 2. Tamoxifen caused the retention of estradiol receptor in nucleus over 24 hours and inhibited the replenishment of the receptor from nucleus to cytosol in uterus. |
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