Korean Journal of Veterinary Research 1986;26(1):31-37.
Study on Antiestrogenic Effects of Tamoxifen in Immature Rat Uterus: II. Effects on Synthesis of Ribonucleic Acid and Protein
Hyo-jong Lee1, Choong-ho Jo2, Moo-hyun Park1
1Department of Veterinary Medicine, College of Agriculture, Gyeongsang National University
2College of Veterinary Medicine, Seoul National University
미성숙 쥐 자궁에서 Tamoxifen의 Antiestrogen 효과에 관한 연구 : II. Ribonucleic Acid 및 단백질 합성능력에 관하여
이효종1, 조충호2, 박무현1
1경상대학교 농과대학 수의학과
2서울대학교 수의과대학
Abstract
The present study has been carried out to elucidate the antiestrogenic effects of tamoxifen on RNA and protein synthesis in uteri of immature rats. Immature female Sprague-Dawley rats were allocated into 4 groups and injected with $5{mu}g$ of estradiol-$17{eta}$, $50{mu}g$ of tamoxifen, a combination of both, or vehicle only subcutaneously three times with an interval of 24 hours respectively. The specific activities of $^3H$-uridine incorporation into uterine RNA and those of $^3H$-leucine incorporation into uterine protein were measured before and 1, 3, 6, 12, 24, 48 and 72 hours after the above treatments. The results obtained were summarized as follows; 1. Tamoxifen itself increased RNA synthesis an hour after treatment(169.18% of control), but it's specific activity was reduced to control level after 3 hours. Tamoxifen inhibited significantly (p<0.01) the activity of RNA synthesis of estradiol-$17{eta}$. 2. The increasing rate of protein synthesis was lower in tamoxifen treated group than that in estradiol-$17{eta}$ treated group. While the rate was steadily increased up to 357.4% of control by estradiol-$17{eta}$ in 72 hours, tamoxifen itself failed to increase the rate after 24 hours and significantly (p<0.01) inhibited the activity of estradiol-$17{eta}$(-167.4%).


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