| In vitro maturation of porcine follicular oocytes |
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Mi-hee Park1, Hyo-jong Lee2 |
1Gyeongnam Veterinary service Laboratory
2College of Veterinary Medicine, Gyeongsang National University |
| 돼지 난포내 난모세포의 체외성숙에 관하여 |
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박미희1, 이효종2 |
1경상남도 가축위생시험소
2경상대학교 수의과대학 |
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| Abstract |
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This experiment was carried out to establish an effective technique of in vitro maturation of porcine follicular oocytes. Porcine ovaries were collected from an abbatoir and delivered to the laboratory in phosphate buffered saline in an hour. Immatured follicular oocytes were collected from the ovaries and divided into groups by the size of follicles and by the attachment of granulosa cells. The follicular oocytes were cultured in m-KRB solution supplemented with FCS(10%), follicular fluid(10%) or hormones of PMSG(10IU/ml), hCG(10IU/ml ) and $estradiol-17{eta}(1{mu}g/ml)$ for 48 hours at $39^{circ}C$ under an atmosphere of 5% $CO_2$ in air. The results are as follows ; 1. The mean recoveration rate of follicular oocytes was 61.8%. 2. The maturation rate was significantly(p<0.05) higher when the oocytes were collected from large-sized follicles and under good state of granulosa cell attachment. 3. The maturation rate was significantly(p<0.01) promoted when the follicular oocytes were cultured in m-KRB solution supplemented with follicular fluid(74.8%) or hormones and fetal calf serum(70.6%). |
| Key Words:
Follicular oocyte, in vitro maturation, pig |
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