Isolation of adenovirus from porcine alveolar macrophages |
Chang-hee Kweon1, Jae-jin Cho1, Byung-joon Kwon1, Jae-young Song1, Chanhee Chae2 |
1National Veterinary Research Institute 2College of Veterinary Medicine Seoul National University |
돼지 폐포탐식세포로부터 adenovirus의 국내분리주 작성 |
권창희1, 조재진1, 권병준1, 송재영1, 채찬희2 |
1수의과학연구소 2서울대학교 수의과대학 |
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Abstract |
One cytopathogenic virus was isolated in alveolar macrophages from pig without any apparent respiratory clinical signs. Biophysical properties and electron microscopy of the isolate showed the characteristics of adenovirus. Intracytoplasmic inclusion bodies were seen in virus-inoculated cells. The genetic analysis indicated the presence of DNA with the size of >20Kb. In a serological survey of 40 serum samples collected from two different farms in slaughter house, 9 sera were positive for neutralizing antibody against the isolate. The potential implications of the isolate as the causative agent in respiratory disorder were discussed. |
Key Words:
adenovirus, porcine alveolar macrophage, biophysical characteristics, serological survey in Korea |
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