| Pathological findings and virus detection by in situ hybridization in the Korean native goats experimentally infected with Aujeszky's disease virus |
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Soon-bok Kim1, Geun-suk Song1, Oun-kyong Moon2, Chang-geun Jeong3 |
1College of Veterinary Medicine, Gyeongsang National University
2Kyongnam Veterinary Service Laboratory
3Air Medical Group, Air Training Command |
| 오제스키병바이러스 인공감염 한국재래산양의 병리학적 소견 및 절편내 in situ hybridization 바이러스 동정 |
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김순복1, 송근석1, 문운경2, 정창근3 |
1경상대학교 수의과대학
2경남가축위생시험소
3공군교육사령부 항공의무전대 |
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| Abstract |
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Aujeszky's disease virus(ADV) was inoculated intranasally into the Korean native goats to investigate pathological findings and pathogenesis of ADV infection by using of histological and immunohistochemical methods and in situ hybridization(ISH). Clinical signs of salvation, pyrexia, pruritus and staggering were followed by death with five days after inoculation, Pathoanatomical findings were edema of the lung and the urinary bladder with hemorrhage and congestion, petechial hemorrhages on the endo-and epicardium, renal congestion, moderate splenomegaly and cystic edema. Main microsocpic lesions observed in all infected goats were confined to the CNS and charcterized by perivascular cuffing with lymphocytes and macrophages, focal gliosis, neuronal degeneration and necrosis, and intranuclear inclusion bodies in the neurons and glial cells. Positive reactions to ADV were detected more frequently in the nuclei than in the cytoplasms of infected nerve cells in the CNS by immunohistochemistry and ISH. Frequenctly localized sites of ADV in the CNS were olfactory bulb, prietal cortex, callosal sulcus and corpus callosum. Positive reactions were also detected in the tonsillar epithelium, and alveolar macrophage and sloughed epithelium of the lung. |
| Key Words:
pathology, immunohistochemistry, in situ hybridization, Aujeszky's disease, Korean natvie goats |
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