| Sequential pathologic changes and viral distribution in rabbits experimentally infected with new Korean strain of rabbit hemorrhagic disease virus (RHDVa) |
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Jung-Won Park1, Ji-Eun Chun1, Dong-Kun Yang1, Eun-Jung Bak2, Han Kim1, Myeong-Heon Lee1, Eui-Kyung Hwang3, Chung-Bok Lee4, Gye-Hyeong Woo5 |
1Animal, Plant and Fisheries Quarantine and Inspection Agency
2Oral Cancer Research Institute, College of Dentistry, Yonsei University
3Department of Animal Science, College of Life Science and Natural Resources, Sangji University
4Department College of Veterinary Medicine, Konkuk University
5Department of Clinical Laboratory Science, Semyung University |
| 새로운 국내 분리 토끼출혈병바이러스(RHDVa)를 감염시킨 토끼에서의 경시적인 병리학적 변화와 조직 내 바이러스 항원 분포 |
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박중원1, 전지은1, 양동군1, 박은정2, 김한1, 이명헌1, 황의경3, 이중복4, 우계형5 |
1농림수산검역검사본부
2연세대학교 치과대학
3상지대학교 동물자원학과
4건국대학교 수의학과
5세명대학교 임상병리학과 |
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| Abstract |
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Rabbit hemorrhagic disease is a highly acute and fatal viral disease caused by rabbit hemorrhagic disease virus (RHDV). Since first outbreak in Korea 1987, RHDV has been continually affected in the country, but the pattern of outbreak seem to be changed. In this study, to understand the pathogenesis of the new RHDVa serotype, we therefore carried out to inoculate RHDVa to rabbits, and to examine the sequential histopathologic changes and viral distribution. Macroscopically, various sized dark red or white spots or appearance were observed in the liver, lung, kidney uterus and ureter. In euhanized rabbits, significant pathologic findings such as infiltration of heterophils and mononuclear cells were observed at 24 hours after inoculation (HAI), and these were sequentially extended periportal to centrilobular area. However, in dead rabbits, severe hepatic degeneration and/or necrosis with relatively weak inflammatory responses were observed. RHDV antigens began to detect in liver, spleen, and lung from 12 HAI by PCR. Immunohistochemically, RHDV positive cells were seen in only liver from 24 HAI, and the degree of immunogen reactivity was stronger in dead rabbits than in euthanized ones. In conclusion, RHDVa caused the subacute or chronic infection accompanying low mortality and moderate to severe inflammatory reaction in rabbits, suggesting the possibility that RHD could become endemic. |
| Key Words:
chronic infection, histopathologic changes, new RHDVa serotype, rabbit hemorrhagic disease, viral distribution |
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