| Characterization of infectious bursal disease viruses isolated in Korea using RT/PCR and RFLP analysis |
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Hyuk-moo Kwon1, Dae-kyu Kim1, Hwan-woo Seong2 |
1Department of Veterinary Medicine, Kangwon National University
2National Veterinary Research and Quarantine Service |
| RT/PCR과 RFLP 분석에 의한 Infectious bursal disease virus(국내분리주)의 특성 규명 |
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권혁무1, 김대규1, 성환우2 |
1강원대학교 수의학과
2국립수의과학검역원 |
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| Abstract |
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Field infectious bursal disease viruses (IBDVs) were isolated from IBDV-suspected commercial chickens. The variable region in VP2 gene of six Korean IBDV isolates (K-IBDVs) and IBD vaccines was examined using the reverse transcriptase / polymerase chain reaction-restriction fragment length polymorphism (RT/PCR-RFLP) assay. With all K-IBDVs and vaccine IBDVs, a 474-bp fragment of the VP2 gene was amplified and tested with various restriction enzymes. Restriction enzymes BstNI and StyI differentiated K-IBDV isolates and IBD vaccines into four groups. Restriction enzyme profiles of K-IBDV isolates were different from them of IBD vaccines. K-IBDV isolates except for 310 isolate had specific SspI and TaqI recognition sites, which were recognized in highly virulent IBDVs, but IBD vaccines had no those sites. This study showed that RT/PCR-RFLP assay was thought to be valuable tool for differentiation of IBDVs and identification of highly virulent IBDV. |
| Key Words:
infectious bursal disease virus, VP2 gene, RT/PCR-RFLP |
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