| Sequence analysis of VP2 gene of infectious bursal disease virus field isolate and vaccine strains |
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Ji-Dong Jin1, Zheng-Wu Kang1, Sun-Joong Kim2, Hyuk-Moo Kwon1, Tae-Wook Hahn1 |
1School of Veterinary Medicine, Kangwon National University
2College of Veterinary Medicine, Seoul National University |
| Infectious bursal disease virus 국내분리주 및 백신주의 VP2 gene의 비교분석 |
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김길동1, 강정무1, 김선중2, 권혁무1, 한태욱1 |
1강원대학교 수의학부
2서울대학교 수의과대학 |
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| Abstract |
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The VP2 full gene of Korean infectious bursal disease virus(IBDV) strain, SH/92, three attenuated vaccine strains, Bur706, Bursine-2 and CEV/AC strains, were amplified by reverse transcriptase-polymerase chain reaction and sequenced and compared with published VP2 gene sequences of IBDVs. The VP2 nucleotide sequence similarity between SH/92 and three vaccine stains was 95.6~96.5% whereas the nucleic acid similarity among three vaccine strains was 97.5~98.5%. The amino acid sequence similarity of VP2 of SH/92 compared with three vaccine strains was between 94.4 and 97.6% while the amino acid similarity among three vaccine strains was between 97.4 and 98.4%. The amino acid similarity between SH/92 and classical virulent strain, 52/70 and STC strain was 96.4 and 96.5%, respectively. The serine-rich heptapeptide was conserved in CEVAC and Bursine-2 as well as SH/92 but not in Bur706. The phylogenetic tree developed from amino acid sequences showed that SH/92 was categorized with vv IBDVs(HK46, OKYM, KKI, UPM94/273, SH95) in one branch while three vaccine strains were catagorized with STC strain in the other branch. |
| Key Words:
Infectious bursal disease virus (IBDV), VP2 gene, vaccine strain, sequence |
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