Protective effects of mix-crude outer membrane protein Salmonella vaccine against salmonellosis in chickens and pigs

Lee, Hee-Soo; Lim, Suk-Kyung; Cho, Yun-Sang; Joo, Yi-Seok; Kim, Jae-Hak; Kim, Jong-Man

Korean Journal of Veterinary Research 2007;47(2):147-155.

Hee-Soo Lee, Suk-Kyung Lim, Yun-Sang Cho, Yi-Seok Joo, Jae-Hak Kim, Jong-Man Kim

National Veterinary Research and Quarantine Services

살모넬라 세포외막단백질 혼합백신을 이용한 돼지 및 닭에서의 살모넬라균감염증 방어효과

이희수, 임숙경, 조윤상, 주이석, 김재학, 김종만

국립수의과학검역원

Abstract

We investigated the safety, immunogenicity and protectivity of mix-crude outer membrane protein (cOMP) vaccine against salmonellosis in animals. The mix-cOMP vaccine was extracted from Salmonella enterica serovar Typhimurium (ST) and Salmonella enterica serovar Enteritidis (SE) and Salmonella enterica serovar Braenderup (SB) isolated from pigs. The mix-cOMP vaccine gave significantly higher antibody response than ST-bacterin and ST-cOMP vaccine in guinea pigs. The survival rates of mix-cOMP vaccinated groups showed significantly higher (100%) than those (0-20%) of unvaccinated control group, challenged with 3 species of Salmonella (ST, SE and SB) in mice. Vaccinated groups in pigs showed reduction of clinical signs, increase of average weight gains, decrease of bacterial recovery rates, compared with unvaccinated groups. Especially, the survival rates (100%) of vaccinated groups in chickens showed higher than that (0%) of unvaccinated group. Based on these results, we suggest that the mix-cOMP Salmonella vaccine developed in this study will be effective for the protection against Salmonellosis caused by the various serotypes Salmonella species in animals.

Key Words: mix-cOMP vaccine, S. braenderup, S. enteritidis, S. typhimurium, samonellosis