| Comparative serological analysis of outer membrane proteins extracted from Brucella abortus Korean isolates and 1119-3 strains |
|
Seung-Bin Cha1, Mi-Lan Kang1, Won-Jung Lee1, Min-Kyoung Shin1, Dong-Hee Cho2, Suk-Chan Jung2, Han-Sang Yoo1 |
1College of Veterinary Medicine, BK 21 Program for Veterinary Science and KRF Zoonotic Diseases Institute, Seoul National University
2National Veterinary Reasearch Institute, National Veterinary Reasearch & Quarantine Service |
| Brucella abortus 국내 분리주의 세포외막 단백질 분석 및 혈청학적 비교 |
|
차승빈1, 강미란1, 이원정1, 신민경1, 조동희2, 정석찬2, 유한상1 |
1서울대학교 수의과대학, BK21 수의과학인력양성 사업단, 인수공통질병연구소
2국립수의과학검역원 동물위생연구소 |
|
|
| Abstract |
|
Brucellosis is one of the most important zoonosis in worldwide. As one of the control measures, attempts have been made to develop new diagnostic methods using filed isolates as a national policy in many countries. Currently, bovine brucellosis in Korea have been received attention in both public health and economical aspects due to sudden increase of outbreak. Based on the situation, we compared standard strain (B. abortus 1119-3) with field isolates to reveal the differences among them. Biological and biochemical charateristics, antibiotic resistance profiles, outer membrane proteins (OMPs) and lipopolysaccharide analysis of the strains were included in this study. For the diagnostic purpose, an attempt was made to find out a novel antigen from the Korean isolates by serological analysis. There were differences about 55 kDa, 36-38 kDa and 20 kDa in analysis of OMPs by SDS-PAGE and Western blot with positive sera ($geq$ 1:400 in SAT titer). Also, a serological diagnostic method, ELISA was conducted using OMPs of the strains as novel antigen. Relationships between O.D. and SAT titer were analyzed using field sera showing different SAT titer. High correlation coefficient was observed between SAT titer and ELISA. Results from this study suggested that a new diagnsotic method should be developed using their own field isolates in each country. |
| Key Words:
Brucella abortus, ELISA, Korean isolates, OMPs |
|
PDF Links
Full text via DOI
Download Citation
