Studies on Vaginal Cytology after Induction of Estrus in the Bitch

Kang, Byong-kyu; Choi, Han-sun; Na, Jin-su; Son, Chang-ho; Oh, Ki-seok; Lee, Cha-bum

Korean Journal of Veterinary Research 1988;28(1):203-211.

Byong-kyu Kang¹, Han-sun Choi¹, Jin-su Na², Chang-ho Son¹, Ki-seok Oh¹, Cha-bum Lee¹

¹College of Veterinary Medicine, Chonnam National University
²Department of Animal Science, College of Agriculture, Chonnam National University

인공적(人工的) 발정유기견(發情誘起犬)의 질세포상(膣細胞像)에 관(關)한 연구(硏究)

강병규¹, 최한선¹, 나진수², 손창호¹, 오기석¹, 이차범¹

¹전남대학교 수의과대학
²전남대학교 농과대학 축산학과

Abstract

The present study was carried out to investigate the estrous behavior and vaginal smear after induction of estrus with exogenous hormones in the premature, metestrous and anestrous bitches. A total of 21 bitches(Mixed breed: 16, Jindo breed: 5), from 10 months to 5 years of age and weighing 8 to 15 kg was studied the change of vaginal smear and the estrous behavior before and after induction of estrus. The results obtained are as follows: 1. In the treatment A(They were given the $PGF_2{alpha}$, estrone, estradiol-$17{eta}$, PMSG and HCG) proestrus commenced in $10.16{pm}1.44$($Mean{pm}SEM$) days after treatment, The mean duration of proestrus, and estrus was $7.50{pm}1.44$ and $13.50{pm}3.44$ days, respectively. In the treatment B(They were given the PMSG and HCG) proestrus commenced in $5.53{pm}0.59$ days after treatment. The mean duration of proestrus and estrus was $6.60{pm}0.71$ and $14.60{pm}1.14$ days, respectively. 2. All of the 6 bitches in the treatment A showed vulval swelling and vaginal discharge, 14 of the 15 bitches in the treatment B showed vulval swelling and vaginal discharge. However, all of the treatment A and B showed male acceptance. 3. The main change of vaginal smear in proestrus and estrus after induction of estrus was a increase in the proportion of anuclear and superficial cells associated with a decrease in small intermediate and parabasal cells. By the estrous behavior and vaginal smear the estrus was induced in all the premature, metestrous and anestrous bitches.